In the very next event on the track, Bol suffered the same devastating fate - this time even closer to glory.
Locked side by side with American Alexis Holmes, the 23-year-old took a hard fall mere metres from the finish line.
Hitting her head against the track after stumbling forward, she dropped her team's baton, meaning a dazed Bol and the rest of the Dutch quartet were disqualified.
"To be honest I don't have a clue [what happened]," Bol told BBC Sport.
"I've had it once before. I'm really sorry for my team. I should finish it off.
"I have tired legs, I think I've had worse tired legs. It happened and it sucks big time."
Holmes, who did not appear to make contact with her rival, completed a world record run by the USA team, with Great Britain and the Czech Republic benefiting from Bol's misfortune.
"I'm still in shock, my heart is pounding. I can't believe we have just seen that back-to-back," said Jessica Ennis-Hill.
Fellow former world heptathlon champion Denise Lewis added: "You just couldn't write it, could you?
"Bol and Hassan are both medal winners. The incidents were almost carbon copies of each other. It is just incredible."
Reflecting on the scarcely believable conclusion to the opening day for the Dutch team, BBC commentator Andrew Cotter said: "What a great run by the American quartet - but this is a dark day for the Netherlands."
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